Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Western blot analysis of Tom20 expression in non-transfected 293T: (A), human Tom20 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Tom20 expression in non-transfected 293T: (A), mouse Tom20 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Tom20 expression in non-transfected 293T: (A), human Tom20 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Tom20 expression in non-transfected: (A) and human Tom20 transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Tom20 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Tom20 (FL-145): .Application
Western blot analysis of Tom20 expression in HeLa (A) and MM-142 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor (B) showing cytoplasmic staining.| Product Name | Tom20 Antibody (FL-145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-145 representing full length Tom20 of human origin |
| Modification Notes | The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane (1). The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria (2). The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP (3,4). In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria (6,7). The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes (2,8) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Tom20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-145 representing full length Tom20 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-145 representing full length Tom20 of human origin |
| Entrez Gene | 9804 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Western blot analysis of Tom20 expression in non-transfected 293T: (A), human Tom20 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Tom20 expression in non-transfected 293T: (A), mouse Tom20 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Tom20 expression in non-transfected 293T: (A), human Tom20 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Tom20 expression in non-transfected: (A) and human Tom20 transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Tom20 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Tom20 (FL-145): .
Application
Western blot analysis of Tom20 expression in HeLa (A) and MM-142 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor (B) showing cytoplasmic staining.| Application Notes | recommended for detection of Tom20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
