Application
Western blot analysis of TRP2 expression in non-transfected: (A) and human TRP2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TRP2 expression in A-375 (A), Y79 (B), CTLL-2 (C) and WEHI-231 (D) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tumor showing cytoplasmic localization.Application
Western blot analysis of TRP2 expression in SK-MEL-28 whole cell lysate.| Product Name | TRP2 Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 41-190 of TRP2 of human origin |
| Modification Notes | Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme, is involved in the production of melanin, the primary pigment found in vertebrates. Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exists as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TRP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 41-190 of TRP2 of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 41-190 of TRP2 of human origin |
| Entrez Gene | 1638 |
|---|
Application
Western blot analysis of TRP2 expression in non-transfected: (A) and human TRP2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TRP2 expression in A-375 (A), Y79 (B), CTLL-2 (C) and WEHI-231 (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tumor showing cytoplasmic localization.
Application
Western blot analysis of TRP2 expression in SK-MEL-28 whole cell lysate.| Application Notes | recommended for detection of TRP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
