Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining of hepatocytes.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and SOD-1 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with SOD-1 (FL-154): .Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.Application
Western blot analysis of SOD-1 expression in HeLa (A), Jurkat (B), Hs68 (C) and DU I45 (D) whole cell lysates.| Product Name | SOD-1 Antibody (FL-154) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-154 representing full length SOD-1 (superoxide dismutase-1) of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Cu-Zn superoxide dismutase-1 (SOD-1) is a well characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer’s disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC-p:1:10~1:50 ELISA:1:1000~1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-154 representing full length SOD-1 (superoxide dismutase-1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-154 representing full length SOD-1 (superoxide dismutase-1) of human origin |
| Entrez Gene | 6647 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining of hepatocytes.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and SOD-1 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with SOD-1 (FL-154): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Application
Western blot analysis of SOD-1 expression in HeLa (A), Jurkat (B), Hs68 (C) and DU I45 (D) whole cell lysates.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC-p:1:10~1:50 ELISA:1:1000~1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
