Application
Western blot analysis of Rac 1 expression in non-transfected 293T: (A), mouse Rac 1 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed rat embryo fibroblasts using fluorescein-labeled goat anti-rabbit IgG secondary antibody.Application
Western blot analysis of human recombinant Rac 1.| Product Name | Rac 1 Antibody (C-14) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Rac 1 of human origin |
| Modification Notes | A large number of low molecular weight, GTP binding proteins of the Ras superfamily have been identified. These proteins regulate many fundamental processes in all eukaryotic cells such as growth, vesicle traffic and cytoskeletal organization. GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. Two proteins in this family, Rac 1 and Rac 2, are 92% identical and share GTP binding and GTP hydrolysis motifs with other members of the Ras superfamily. Rac 1 is expressed in a large number of different cell types. Rac 2 is primarily expressed only in myeloid cells and has been reported to be a regulatory component of the human neutrophil NADPH oxidase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rac 1 and, to a lesser extent, Rac 3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Rac 2 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Rac 1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Rac 1 of human origin |
| Entrez Gene | 5879 |
|---|
Application
Western blot analysis of Rac 1 expression in non-transfected 293T: (A), mouse Rac 1 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed rat embryo fibroblasts using fluorescein-labeled goat anti-rabbit IgG secondary antibody.
Application
Western blot analysis of human recombinant Rac 1.| Application Notes | recommended for detection of Rac 1 and, to a lesser extent, Rac 3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Rac 2 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
