Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Antibodies tested include Ac-Histone H2B (Lys 5/12/15/20): (A,B) and Histone H2B (FL-126): (C,D).Application
Antibodies tested include Ac-Histone H2B (Lys 5/12/15/20)-R: (A,B) and Histone H2B (FL-126): (C,D).Application
Antibodies tested include p-Histone H2B (Ser 14): (A,B,C) and Histone H2B (FL-126): (D,E,F).Application
Antibody tested is Histone H2B (FL-126): (A,B).Application
Western blot analysis of Ac-Histone H2B acetylation in untreated (A,C) and Trichostatin A treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include: Ac-Histone H2B (Lys 5/12/15/20): (A,B) and Histone H2B (FL-126): (C,D).Application
Antibodies tested include: Ac-Histone H2B (Lys 5/12/15/20)-R: (A,B) and Histone H2B (FL-126): (C,D).Application
Western blot analysis of Histone cluster 2 H2BE expression in non-transfected: (A) and mouse Histone cluster 2 H2BE transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Histone cluster 1 H2BK expression in non-transfected: (A) and human Histone cluster 1 H2BK transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Histone H2B expression in HeLa whole cell lysate.Application
Western blot analysis of Histone cluster 1 H2BK expression in non-transfected 293T: (A), human Histone cluster 1 H2BK transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Histone cluster 1 H2BK expression in non-transfected 293T: (A) and human Histone cluster 1 H2BK transfected 293T: (B) whole cell lysates and BJAB nuclear extract (C).| Product Name | Histone H2B Antibody (FL-126) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-126 representing full length Histone H2B of human origin |
| Modification Notes | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Histone H2B of broad mammalian, Drosophila, Xenopus and C. elegans origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-126 representing full length Histone H2B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-126 representing full length Histone H2B of human origin |
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Antibodies tested include Ac-Histone H2B (Lys 5/12/15/20): (A,B) and Histone H2B (FL-126): (C,D).
Application
Antibodies tested include Ac-Histone H2B (Lys 5/12/15/20)-R: (A,B) and Histone H2B (FL-126): (C,D).
Application
Antibodies tested include p-Histone H2B (Ser 14): (A,B,C) and Histone H2B (FL-126): (D,E,F).
Application
Antibody tested is Histone H2B (FL-126): (A,B).
Application
Western blot analysis of Ac-Histone H2B acetylation in untreated (A,C) and Trichostatin A treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include: Ac-Histone H2B (Lys 5/12/15/20): (A,B) and Histone H2B (FL-126): (C,D).
Application
Antibodies tested include: Ac-Histone H2B (Lys 5/12/15/20)-R: (A,B) and Histone H2B (FL-126): (C,D).
Application
Western blot analysis of Histone cluster 2 H2BE expression in non-transfected: (A) and mouse Histone cluster 2 H2BE transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Histone cluster 1 H2BK expression in non-transfected: (A) and human Histone cluster 1 H2BK transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Histone H2B expression in HeLa whole cell lysate.
Application
Western blot analysis of Histone cluster 1 H2BK expression in non-transfected 293T: (A), human Histone cluster 1 H2BK transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Histone cluster 1 H2BK expression in non-transfected 293T: (A) and human Histone cluster 1 H2BK transfected 293T: (B) whole cell lysates and BJAB nuclear extract (C).| Application Notes | recommended for detection of Histone H2B of broad mammalian, Drosophila, Xenopus and C. elegans origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
