Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in distal tubules.Application
Western blot analysis of THP expression in non-transfected: (A) and mouse THP transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and extracellular localization.Application
Western blot analysis of human recombinant THP fusion protein.| Product Name | THP Antibody (H-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 291-425 mapping within an internal region of Tamm-Horsfall urinary glycoprotien (THP) of human origin |
| Modification Notes | Tamm-Horsfall glycoprotein (also referred to as uromodulin or THP) is the most abundant protein found in normal urine (1–3). THP is expressed on the luminal surface of the membrane with the glycosyl phosphatidylinositol (GPI) anchor and excreted in urine at a rate of 50–100 mg per day (4). THP, uropontin and nephrocalcin are the three known urinary glycoproteins that affect the formation of calcium-containing kidney stones (1). THP is synthesized by kidney epithelial cells and is believed to play important and diverse roles in the urinary system, including renal water balance, immunosuppression, urinary stone formation and inhibition of bacterial adhesion (5). THP is nontoxic and blocks early events required for normal T-cell proliferation in vitro (3). The gene which encodes THP and is a candidate gene for nephrolithiasis maps to human chromosome 16p13.11. (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of THP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 291-425 mapping within an internal region of Tamm-Horsfall urinary glycoprotien (THP) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 291-425 mapping within an internal region of Tamm-Horsfall urinary glycoprotien (THP) of human origin |
| Entrez Gene | 7369 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in distal tubules.
Application
Western blot analysis of THP expression in non-transfected: (A) and mouse THP transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and extracellular localization.
Application
Western blot analysis of human recombinant THP fusion protein.| Application Notes | recommended for detection of THP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
