Application
Western blot analysis of Eps15 expression in non-transfected 293T: (A), mouse Eps15 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.Application
Western blot analysis of Eps15 expression in NIH/3T3 whole cell lysate.| Product Name | Eps15 Antibody (H-896) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-896 representing full length Eps15 of human origin |
| Modification Notes | Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer (1,2). Two novel EGF receptor substrates designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15, have been described (3,4). Eps8 and Eps15 become tyrosine phosphorylated subsequent to EGF stimulation (3,4). Overexpression of Eps15 in NIH/3T3 cells causes cellular transformation, implying involvement in the regulation of cell proliferation (4). Eps15 is capable of binding the amino terminal portion of Crk via a conserved proline-rich domain, characteristic of all Crk binding proteins (5). Overexpression of Eps8 in both fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF (3). Eps8 has been shown to associate with the EGF receptor despite its lack of a functional SH2 domain (6). Further characterization suggests the protein has both a PH domain and a SH3 domain, the functional significance of which are not yet known (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Eps15 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-896 representing full length Eps15 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-896 representing full length Eps15 of human origin |
| Entrez Gene | 2060 |
|---|
Application
Western blot analysis of Eps15 expression in non-transfected 293T: (A), mouse Eps15 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.
Application
Western blot analysis of Eps15 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of Eps15 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
