Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human invasive ductal primary mammary carcinoma tissue. Kindly provided by Raffaella Muraro.Application
Western blot analysis of ErbB receptor expression in NIH/3T3 transfectants expressing between 105-106 molecules/cell of ErbB-2 (A), ErbB-3 (B) and ErbB-4 (C) and in NIH/3T3 (D) whole cell lysate.Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-4 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-4 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal goat IgG: .Application
Western blot analysis of non-transfected (A) and ErbB-4-transfected (B) NIH/3T3 cells.| Product Name | ErbB-4 Antibody (C-18) -G |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of ErbB-4 of human origin |
| Modification Notes | The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of ErbB-4 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of ErbB-4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of ErbB-4 of human origin |
| Entrez Gene | 2066 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human invasive ductal primary mammary carcinoma tissue. Kindly provided by Raffaella Muraro.
Application
Western blot analysis of ErbB receptor expression in NIH/3T3 transfectants expressing between 105-106 molecules/cell of ErbB-2 (A), ErbB-3 (B) and ErbB-4 (C) and in NIH/3T3 (D) whole cell lysate.
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-4 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-4 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal goat IgG: .
Application
Western blot analysis of non-transfected (A) and ErbB-4-transfected (B) NIH/3T3 cells.| Application Notes | recommended for detection of ErbB-4 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
