Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of urothelial cells.Application
Western blot analysis of mSin3A expression in non-transfected 293T: (A), human mSin3A transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of mSin3A expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.| Product Name | mSin3A Antibody (AK-11) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 322-381 mapping within the PAH2 region of mSin3A of mouse origin. |
| Modification Notes | It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (alternatively designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of mSin3A and, to a lesser extent, mSin3B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 322-381 mapping within the PAH2 region of mSin3A of mouse origin. |
|---|---|
| Tissue Specificity | raised against amino acids 322-381 mapping within the PAH2 region of mSin3A of mouse origin. |
| Entrez Gene | 25942 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of urothelial cells.
Application
Western blot analysis of mSin3A expression in non-transfected 293T: (A), human mSin3A transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of mSin3A expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.| Application Notes | recommended for detection of mSin3A and, to a lesser extent, mSin3B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
