Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of Flt-1 expression in non-transfected: (A) and truncated human Flt-1 transfected: (B) 293T whole cell lysates.| Product Name | Flt-1 Antibody (H-225) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 23-247 mapping within the extracellular domain of Flt-1 of human origin |
| Modification Notes | Three cell membrane receptor tyrosine kinases, Flt-1 (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt-1 and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Flt-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 23-247 mapping within the extracellular domain of Flt-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 23-247 mapping within the extracellular domain of Flt-1 of human origin |
| Entrez Gene | 2321 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of Flt-1 expression in non-transfected: (A) and truncated human Flt-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Flt-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
