Application
Western blot analysis of WISP-3 expression in Caki-1 (A) and F9 (B) whole cell lysates.Application
Western blot analysis of WISP-3 expression in non-transfected 293T: (A), human WISP-3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of WISP-3 expression in COLO 320DM (A) and F9 (B) whole cell lysates and rat testis tissue extract (C).| Product Name | WISP-3 Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 231-295 of WISP-3 of human origin |
| Modification Notes | Wnt-induced secreted protein (WISP)-1, WISP-2 and WISP-3 are members of the CCN family of growth factors, which include connective tissue growth factor (CTGF) and Cyr61. WISP-1, WISP-2 and WISP-3 share significant sequence similarity, including four conserved cysteine-rich domains, and they are believed to function as dimers in their active forms. WISP-1 expression is observed in various tissues including adult heart, kidney and spleen, while WISP-2 expression predominates in skeletal muscle, colon and ovary. Both WISP-1 and WISP-2 are upregulated in cells transformed with the proto-oncogene Wnt-1, and they are also more highly expressed in human colon tumors, suggesting that these proteins may participate in tumor development. WISP-3 is involved in normal post-natal skeletal growth, and it is also implicated in the development of the autosomal recessive skeletal disorder progressive pseudorheumatoid dysplasia, which affects cartilage homeostasis by disrupting the growth of chondrocyte and normal cell columnar organization. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of WISP-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-295 of WISP-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-295 of WISP-3 of human origin |
| Entrez Gene | 8838 |
|---|
Application
Western blot analysis of WISP-3 expression in Caki-1 (A) and F9 (B) whole cell lysates.
Application
Western blot analysis of WISP-3 expression in non-transfected 293T: (A), human WISP-3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of WISP-3 expression in COLO 320DM (A) and F9 (B) whole cell lysates and rat testis tissue extract (C).| Application Notes | recommended for detection of WISP-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
