Application
Western blot analysis of AMPKγ2 expression in non-transfected: (A) and human AMPKγ2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AMPKγ2 expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual and trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and perinuclear staining.Application
Western blot analysis of AMPKγ2 expression in JAR (A) and C2C12 (B) whole cell lysates.| Product Name | AMPKγ2 Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-260 mapping at the N-terminus of AMPKγ2 of human origin |
| Modification Notes | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic a subunit and regulatory b and g subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKa1 and AMPKa2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKb1 encodes a 271 amino acid protein and human AMPKb2 encodes a 272 amino acid protein. The human AMPKg1 gene encodes a 331 amino acid protein. Human AMPKg2 and AMPKg3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AMPKγ2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-260 mapping at the N-terminus of AMPK&gamma 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-260 mapping at the N-terminus of AMPKγ2 of human origin |
| Entrez Gene | 51422 |
|---|
Application
Western blot analysis of AMPKγ2 expression in non-transfected: (A) and human AMPKγ2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AMPKγ2 expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual and trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and perinuclear staining.
Application
Western blot analysis of AMPKγ2 expression in JAR (A) and C2C12 (B) whole cell lysates.| Application Notes | recommended for detection of AMPKγ2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
