Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of PKAα cat expression in non-transfected: (A) and human PKAα cat transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PKAα cat expression in non-transfected 293T: (A), mouse PKAα cat transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in ductus seminiferus and Leydig cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PKAα catalytic subunit expression in mouse brain extract (A), NIH/3T3 (B), KNRK (C), Mv 1 Lu (D), MDCK (E), MCF7 (F), PC-3 (G) and A549 (H) whole cell lysates.| Product Name | PKAα cat Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PKAα cat of human origin |
| Modification Notes | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase A (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated PKAå cat (Cå), PKA∫ cat (C∫) and PKA© cat (C©). Each subunit represents specific gene products. PKAå cat and PKA∫ cat are closely related (93% amino acid sequence similarity), whereas PKA© cat displays 83% and 79% similarity to PKAå cat and PKA∫ cat, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PKAα catalytic subunit of mouse, rat, human, canine and mink origin by WB, IP, IF, IHC(P) and ELISA partially cross-reactive with β and γ subunits also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKA&alpha cat of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKAα cat of human origin |
| Entrez Gene | 5566 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of PKAα cat expression in non-transfected: (A) and human PKAα cat transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PKAα cat expression in non-transfected 293T: (A), mouse PKAα cat transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in ductus seminiferus and Leydig cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PKAα catalytic subunit expression in mouse brain extract (A), NIH/3T3 (B), KNRK (C), Mv 1 Lu (D), MDCK (E), MCF7 (F), PC-3 (G) and A549 (H) whole cell lysates.| Application Notes | recommended for detection of PKAα catalytic subunit of mouse, rat, human, canine and mink origin by WB, IP, IF, IHC(P) and ELISA partially cross-reactive with β and γ subunits also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
