Application
Western blot analysis of PRAK expression in non-transfected: (A) and mouse PRAK transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PRAK expression in non-transfected 293T: (A), human PRAK transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | PRAK Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 294-473 mapping at the C-terminus of PRAK of human origin |
| Modification Notes | PRAK (p38-regulated/activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is a ubiquitously expressed serine/threonine kinase regulated by p38a and p38b MAP kinases (1,2). Activated JNK, p38g or p38d are unable to induce phosphorylation of PRAK in vitro (2). Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines (1,3). Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro (1,4,5). Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PRAK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 294-473 mapping at the C-terminus of PRAK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 294-473 mapping at the C-terminus of PRAK of human origin |
| Entrez Gene | 8550 |
|---|
Application
Western blot analysis of PRAK expression in non-transfected: (A) and mouse PRAK transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PRAK expression in non-transfected 293T: (A), human PRAK transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of PRAK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
