Application
Western blot analysis of LysRS expression in non-transfected 293T: (A), human LysRS transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of LysRS expression in HeLa whole cell lysate.Application
Western blot analysis of LysRS expression in non-transfected 293T: (A), mouse LysRS transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Product Name | LysRS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 22-321 mapping near the N-terminus of LysRS of human origin |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. LysRS (lysyl-tRNA synthetase), also known as KARS, KRS or KARS2, exists as both mitochondrial and cytoplasmic isoforms (625 and 576 amino acids, respectively) that belong to the tRNA synthetase family and are thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. The gene encoding LysRS maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LysRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 22-321 mapping near the N-terminus of LysRS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 22-321 mapping near the N-terminus of LysRS of human origin |
| Entrez Gene | 3735 |
|---|
Application
Western blot analysis of LysRS expression in non-transfected 293T: (A), human LysRS transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of LysRS expression in HeLa whole cell lysate.
Application
Western blot analysis of LysRS expression in non-transfected 293T: (A), mouse LysRS transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of LysRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
