Application
Western blot analysis of Nanos1 expression in T-47D whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | Nanos1 Antibody (K-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Nanos1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Nanos1, also known as NOS1, is a 292 amino acid protein that localizes to the perinuclear region of the cytoplasm and contains one nanos-type zinc finger. Expressed at high levels in spermatogonia and present at lower levels in fetal ovaries, Nanos1 forms a complex with Pumilio 2 and functions to regulate the translation of select mRNAs, specifically via association with the 3’-UTR of its mRNA targets. Additionally, Nanos1 is required for the establishment and maintenance of germline stem cells, as it prevents their premature entry into oogenesis. The gene encoding Nanos1 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Nanos1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Nanos1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 340719 |
|---|
Application
Western blot analysis of Nanos1 expression in T-47D whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
