Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Antibodies tested include p-ASK 1 (Ser 83): (A,B,C) and ASK 1 (H-300): (D,E,F).Application
Antibodies tested include p-ASK 1 (B-5): (A,B,C) and ASK 1 (H-300): (D,E,F).Application
Western blot analysis of ASK 1 expression in non-transfected 293T: (A), human ASK 1 transfected 293T: (B) and BJAB (C) whole cell lysates.Application
Western blot analysis of ASK 1 expression in non-transfected: (A) and human ASK 1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing cytoplasmic staining.Application
Western blot analysis of ASK 11 expression in BJAB whole cell lysate.Application
Western blot analysis of ASK 1 expression in BJAB whole cell lysate.| Product Name | ASK 1 Antibody (H-300) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1076-1375 mapping at the C-terminus of ASK 1 of human origin |
| Modification Notes | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ASK 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1076-1375 mapping at the C-terminus of ASK 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1076-1375 mapping at the C-terminus of ASK 1 of human origin |
| Entrez Gene | 4217 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Antibodies tested include p-ASK 1 (Ser 83): (A,B,C) and ASK 1 (H-300): (D,E,F).
Application
Antibodies tested include p-ASK 1 (B-5): (A,B,C) and ASK 1 (H-300): (D,E,F).
Application
Western blot analysis of ASK 1 expression in non-transfected 293T: (A), human ASK 1 transfected 293T: (B) and BJAB (C) whole cell lysates.
Application
Western blot analysis of ASK 1 expression in non-transfected: (A) and human ASK 1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing cytoplasmic staining.
Application
Western blot analysis of ASK 11 expression in BJAB whole cell lysate.
Application
Western blot analysis of ASK 1 expression in BJAB whole cell lysate.| Application Notes | recommended for detection of ASK 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
