Application
Western blot analysis of VDR expression in non-transfected: (A) and mouse VDR transfected: (B) 293T whole cell lysates.Application
Western blot analysis of VDR expression in SK-BR-3 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear localization.Application
Western blot analysis of human recombinant VDR fusion protein.| Product Name | VDR Antibody (H-81) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 344-424 mapping at the C-terminus of VDR of human origin |
| Modification Notes | The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 344-424 mapping at the C-terminus of VDR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 344-424 mapping at the C-terminus of VDR of human origin |
| Entrez Gene | 7421 |
|---|
Application
Western blot analysis of VDR expression in non-transfected: (A) and mouse VDR transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of VDR expression in SK-BR-3 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear localization.
Application
Western blot analysis of human recombinant VDR fusion protein.| Application Notes | recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
