Application
Antibodies tested include MEK kinase-1 (2-8B-5E): at 1.0 µg/ml (A), 0.1 µg/ml (B) and 0.01 µg/ml (C) and MEK kinase-1 (43-Y): at 0.1 µg/ml (D).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse lymph node tissue showing cytoplasmic localization.| Product Name | MEK kinase-1 Antibody (43-Y) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | produced by immunization with 300 amino acid internal region of human MEK kinase-1 expressed in E. coli as polyhistidine tagged fusion protein |
| Modification Notes | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 activates the ERK and c-Jun NH(2)-terminal kinase (JNK) pathways by phosphorylation of MAP2K1 and MAP2K4, and also activates the central protein kinases of the NF˚B pathway, CHUK and IKBKB. Additionally, MEK kinase-1 uses an E3 ligase through its PHD domain, a RING-finger-like structure, to target proteins for degradation through ubiquitination. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MEK kinase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | produced by immunization with 300 amino acid internal region of human MEK kinase-1 expressed in E. coli as polyhistidine tagged fusion protein |
|---|---|
| Tissue Specificity | produced by immunization with 300 amino acid internal region of human MEK kinase-1 expressed in E. coli as polyhistidine tagged fusion protein |
| Entrez Gene | 4214 |
|---|
Application
Antibodies tested include MEK kinase-1 (2-8B-5E): at 1.0 µg/ml (A), 0.1 µg/ml (B) and 0.01 µg/ml (C) and MEK kinase-1 (43-Y): at 0.1 µg/ml (D).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse lymph node tissue showing cytoplasmic localization.| Application Notes | recommended for detection of MEK kinase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
