Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and mouse Sox-9 transfected: (B) 293T whole cell lysates and SW480 nuclear extract (C).Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and mouse Sox-9 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and mouse Sox-9 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and human Sox-9 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse embryo showing nuclear localization.Application
Western blot analysis of Sox-9 expression in C3H/10T1/2 whole cell lysate.| Product Name | Sox-9 Antibody (H-90) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 407-496 of Sox-9 of human origin |
| Modification Notes | Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Sox-9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 407-496 of Sox-9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 407-496 of Sox-9 of human origin |
| Entrez Gene | 6662 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.
Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and mouse Sox-9 transfected: (B) 293T whole cell lysates and SW480 nuclear extract (C).
Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and mouse Sox-9 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and mouse Sox-9 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Sox-9 expression in non-transfected: (A) and human Sox-9 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse embryo showing nuclear localization.
Application
Western blot analysis of Sox-9 expression in C3H/10T1/2 whole cell lysate.| Application Notes | recommended for detection of Sox-9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
