Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.| Product Name | SGK Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 381-420 mapping near the C-terminus of SGK of human origin |
| Modification Notes | Serum- and glucocorticoid-regulated kinase (SGK), also known as SGK1, is a serine/threonine protein kinase and a member of the "AGC" subfamily, which includes protein kinases A, G, and C. SGK plays an important role in activating certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK contains a catalytic domain, which is most similar to Akt1 (also known as protein kinase B or PKB). SGK is a downstream target of PI 3-kinase-stimulated growth factor signaling, with 3-phosphoinositide-dependent protein kinase 1 (PDK1) capable of phosphorylating the activation-loop of SGK at Threonine-256. The adrenal corticosteroid hormone, Aldosterone, induces the transcription of SGK, which mediates Na + transport by stimulating epithelial sodium channel activity. The SGK promoter contains a glucocorticoid response element and an SP-1 regulatory element, and is a transcriptional target for p53. SGK is also a component of the p38 MAPK-mediated response to hyperosmotic stress. The human SGK gene maps to chromosome 6q23 and encodes the 431-amino acid SGK protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of SGK of mouse, rat and human origin by WB, IP, IF and IHC(P) also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 381-420 mapping near the C-terminus of SGK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 381-420 mapping near the C-terminus of SGK of human origin |
| Entrez Gene | 6446 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.| Application Notes | recommended for detection of SGK of mouse, rat and human origin by WB, IP, IF and IHC(P) also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
