Application
Western blot analysis of WRN expression in KNRK nuclear extract (A) and NAMALWA whole cell lysate (B).Application
Western blot analysis of WRN expression in non-transfected control (A) and WRN siRNA transfected (B) HeLa cells. Blot probed with WRN (H-300): . α-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of WRN expression in K-562 (A) and KNRK (B) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.| Product Name | WRN Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1133-1432 mapping at the C-terminus of Werner’s Syndrome protein (WRN) of human origin |
| Modification Notes | Werner’s Syndrome (WS), also called adult progeria, is an inherited, autosomal recessive disorder that is most common in families from regions of Japan where consanguineous marriages occur frequently (1-3). WS is characterized by premature aging and the early onset of age-related diseases and commonly results in cancer (4,5). The gene responsible for Werner’s Syndrome, WRN, has been mapped to the short arm of chromosome 8, 8p11.2-p12 and the subsequent cloning of the gene has reveled a predicted protein of 1432 amino acids in length, that bears significant sequence homology with DNA helicases (6,7). Four mutations in WRN have been identified in patients afflicted with WS (7). Two of the mutations involve mRNA splice-junctions. Of these two mutations, one was found in 60 percent of the individuals examined (7). This mutation is predicted to cause a frameshift which results in a truncated WRN protein (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of WRN of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1133-1432 mapping at the C-terminus of Werner’s Syndrome protein (WRN) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1133-1432 mapping at the C-terminus of Werner’s Syndrome protein (WRN) of human origin |
| Entrez Gene | 7486 |
|---|
Application
Western blot analysis of WRN expression in KNRK nuclear extract (A) and NAMALWA whole cell lysate (B).
Application
Western blot analysis of WRN expression in non-transfected control (A) and WRN siRNA transfected (B) HeLa cells. Blot probed with WRN (H-300): . α-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of WRN expression in K-562 (A) and KNRK (B) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.| Application Notes | recommended for detection of WRN of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
