Application
Western blot analysis of TFIIE-β expression in non-transfected 293T: (A), mouse TFIIE-β transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of TFIIE-β expression in non-transfected control (A) and TFIIE-β siRNA transfected (B) HeLa cells. Blot probed with TFIIE-β (H-291): . GAPDH (FL-335): used as specificity and loading control.Application
Antibodies tested include TFIIE-β (C-21): (A) and TFIIE-β (H-291): (B).| Product Name | TFIIE-β Antibody (H-291) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-291 representing full length TFIIE-β p34 of human origin |
| Modification Notes | In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits, a and b. The structure of TFIIE appears to be a heterotetramer (å2∫2); both subunits are required for optimal basal-level transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of TFIIE-β p34 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-291 representing full length TFIIE-&beta p34 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-291 representing full length TFIIE-β p34 of human origin |
| Entrez Gene | 2961 |
|---|
Application
Western blot analysis of TFIIE-β expression in non-transfected 293T: (A), mouse TFIIE-β transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of TFIIE-β expression in non-transfected control (A) and TFIIE-β siRNA transfected (B) HeLa cells. Blot probed with TFIIE-β (H-291): . GAPDH (FL-335): used as specificity and loading control.
Application
Antibodies tested include TFIIE-β (C-21): (A) and TFIIE-β (H-291): (B).| Application Notes | recommended for detection of TFIIE-β p34 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
