Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse testis tissue showing membrane and cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed U-937 cells showing cytoplasmic localization.| Product Name | SREBP-2 Antibody (H-164) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 812-975 mapping to an internal region of SREBP-2 of human origin |
| Modification Notes | The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a 10 base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SRE-binding proteins (SREBPs) have been identified, including two basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 and SREBP-2 have been shown to have the same specificity for SRE-1 in vitro and to activate the transcription of reporter genes containing SRE-1 in the same way. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SREBP-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 812-975 mapping to an internal region of SREBP-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 812-975 mapping to an internal region of SREBP-2 of human origin |
| Entrez Gene | 6721 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse testis tissue showing membrane and cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed U-937 cells showing cytoplasmic localization.| Application Notes | recommended for detection of SREBP-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
