Application
Western blot analysis of C/EBP α expression in non-transfected 293T: (A), mouse C/EBP α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of C/EBP α expression in non-transfected 293T: (A), mouse C/EBP α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of C/EBP α expression in non-transfected: (A) and mouse C/EBP α transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.Application
Western blot analysis of C/EBP α expression in rat liver tissue extract.| Product Name | C/EBP α Antibody (14AA) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of C/EBP α p42 of rat origin |
| Modification Notes | The transcription factor C/EBP å (CCAAT-enhancer binding protein) is a heat-stable, sequence-specific DNA-binding protein that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP å regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP å is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP å uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growth-arrested cells. In the liver, C/EBP å is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP å mRNA and protein, but also the DNA binding activity of C/EBP å. C/EBP å functions as an important transcription factor that regulates different genes, including prolactin gene expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of C/EBP α p42 and p30 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of C/EBP &alpha p42 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of C/EBP α p42 of rat origin |
| Entrez Gene | 1050 |
|---|
Application
Western blot analysis of C/EBP α expression in non-transfected 293T: (A), mouse C/EBP α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of C/EBP α expression in non-transfected 293T: (A), mouse C/EBP α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of C/EBP α expression in non-transfected: (A) and mouse C/EBP α transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.
Application
Western blot analysis of C/EBP α expression in rat liver tissue extract.| Application Notes | recommended for detection of C/EBP α p42 and p30 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
