Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of CDP expression in non-transfected: (A) and human CDP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CDP expression in K-562 (A) and BJAB (B) nuclear extracts.| Product Name | CDP Antibody (M-222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1111-1332 mapping at the C-terminus of CDP of mouse origin |
| Modification Notes | CDP (for CCAAT displacement protein) has been identified as a repressor for transcription of developmentally regulated genes. It is a homeodomain protein that appears to compete with transcriptional activating proteins for binding to the promoter regions of various genes. CDP contains three cut repeats which function as DNA binding domains. It has been demonstrated that cut repeat domains have the capacity to bind to DNA in conjunction with or independently of homeodomain DNA binding. CDP has been shown to be the DNA-binding subunit of the HiNF-D complex, which contains cyclin A, Cdc2 and an Rb-related protein, in addition to CDP. Histone expression is required for the transition to S phase in the cell cycle. The HiNF-D complex regulates the transcription of histone H4, H3 and H1 genes, allowing cells to progress from G1 to S phase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of CDP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1111-1332 mapping at the C-terminus of CDP of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1111-1332 mapping at the C-terminus of CDP of mouse origin |
| Entrez Gene | 1523 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of CDP expression in non-transfected: (A) and human CDP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CDP expression in K-562 (A) and BJAB (B) nuclear extracts.| Application Notes | recommended for detection of CDP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
