Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of AP-2β expression in non-transfected: (A) and human AP-2β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AP-2β expression in KNRK whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of AP-2β expression in KNRK nuclear extract.| Product Name | AP-2β Antibody (H-87) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 130-216 mapping within an internal region of AP-2β of human origin |
| Modification Notes | AP-2 transcription factor family members include AP-2å, AP-2∫ and AP-2©, which specifically bind to the DNA consensus sequence CCCCAGGC and initiate transcription of selected genes. AP-2, also known as ERF-1, plays a role in regulating estrogen receptor expression. AP-2∫, a splice variant of AP-2å, inhibits AP-2 activity. Besides subscribing to the AP-2 complex, AP-2å, AP-2∫ and AP-2© proteins compose the OB2-1 transcription factor complex. OB2-1 specifically upregulates expression of the proto-oncogene c-ErbB-2, which is overexpressed in 25-30% of breast cancers. The gene encoding AP-2å maps to human chromosome 6p24. AP-2å may play an important role in the development of ectodermal-derived tissues. Deleterious mutations involving the AP-2å gene are linked to microphthalmia, corneal clouding and other anterior eye chamber defects. The ubiquitously expressed AP-4 transcription factor specifically binds to the DNA consensus sequence 5’-CAGCTG-3’. AP-4 interacts with promoters for immunoglobulin-˚ gene families and simian virus 40. AP-4 may enhance the transcription of the human Huntington’s disease gene. AP-4 is a helix-loop-helix protein that contains two distinctive leucine repeat elements. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of AP-2β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 130-216 mapping within an internal region of AP-2&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 130-216 mapping within an internal region of AP-2β of human origin |
| Entrez Gene | 7021 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of AP-2β expression in non-transfected: (A) and human AP-2β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AP-2β expression in KNRK whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of AP-2β expression in KNRK nuclear extract.| Application Notes | recommended for detection of AP-2β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
