Application
Western blot analysis of yeast recombinant Ras2 fusion protein.| Product Name | Ras2 Antibody (y-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 181-310 mapping near the C-terminus of Ras2 of Saccharomyces cerevisiae origin |
| Modification Notes | The guanine nucleotide exchange factor Cdc25 (also designated Ctn1) regulates adenylyl cyclase via the small G proteins Ras1 and Ras2 (also known as Glc5 or Ctn5) (1,2). The yeast RAS proteins regulate cell growth and development by cycling between an active GTP-bound state and an inactive GDP-bound state (3). Adenylyl cyclase, encoded by the Cdc35 gene (also designated Cyr1, Hrs1 or Sra4), catalyzes the formation of the second messenger cAMP (4,5). cAMP exerts its effects via a cAMP-dependent kinase consisting of two regulatory subunits, encoded by Bcy1 (also designated Reg1 or Sra1), and two catalytic subunits, encoded by Tpk1 (also designated Pka1 or Sra3) (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ras2 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-310 mapping near the C-terminus of Ras2 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-310 mapping near the C-terminus of Ras2 of Saccharomyces cerevisiae origin |
Application
Western blot analysis of yeast recombinant Ras2 fusion protein.| Application Notes | recommended for detection of Ras2 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
