Application
Western blot analysis of ICAD expression in non-transfected 293T: (A), mouse ICAD transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of ICAD expression in non-transfected control (A) and ICAD siRNA transfected (B) HeLa cells. Blot probed with ICAD (FL-331): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of ICAD and partial ICAD expression in HeLa (A) and U-937 (B) whole cell lysates and human recombinant ICAD fusion protein (C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tissue showing nuclear and cytoplasmic staining.| Product Name | ICAD Antibody (FL-331) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-331 representing full length ICAD of human origin |
| Modification Notes | The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ICAD and DFF-35 splice variant of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-331 representing full length ICAD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-331 representing full length ICAD of human origin |
| Entrez Gene | 1676 |
|---|
Application
Western blot analysis of ICAD expression in non-transfected 293T: (A), mouse ICAD transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of ICAD expression in non-transfected control (A) and ICAD siRNA transfected (B) HeLa cells. Blot probed with ICAD (FL-331): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of ICAD and partial ICAD expression in HeLa (A) and U-937 (B) whole cell lysates and human recombinant ICAD fusion protein (C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of ICAD and DFF-35 splice variant of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
