Application
Western blot analysis of survivin expression in non-transfected: (A) and mouse survivin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of survivin expression in non-transfected: (A) and human survivin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of survivin expression in MOLT-4 (A) and HL-60 (B) whole cell lysates.Application
Western blot analysis of human recombinant survivin fusion protein.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.| Product Name | survivin Antibody (FL-142) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-142 of survivin of human origin |
| Modification Notes | The baculovirus protein p35 inhibits virally-induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the immune system of the host. This is accomplished at least in part by the ability of p35 to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FAS-mediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivin-microtubule interactions occurs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of survivin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~1:5000 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-142 of survivin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-142 of survivin of human origin |
| Entrez Gene | 332 |
|---|
Application
Western blot analysis of survivin expression in non-transfected: (A) and mouse survivin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of survivin expression in non-transfected: (A) and human survivin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of survivin expression in MOLT-4 (A) and HL-60 (B) whole cell lysates.
Application
Western blot analysis of human recombinant survivin fusion protein.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.| Application Notes | recommended for detection of survivin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~1:5000 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.Stable for 12 months from date of receipt |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C. |
