Application
Western blot analysis of NF2 expression in non-transfected 293T: (A), mouse NF2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic staining.Application
Western blot analysis of NF2 expression in KNRK whole cell lysate.| Product Name | NF2 Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of NF2 of human origin |
| Modification Notes | Neurofibromatosis type 2 (NF2) is a dominantly inherited disorder characterized by the occurance of bilateral vestibular schwannomas and other central nervous system tumors, including multiple meningiomas. NF2 occurs in about 1 of 40,000 live births. The NF2 gene is highly penetrant; NF2-affected individuals have a 95% chance of developing bilateral vestibular schwannomas. NF2 is distinct from NF1, which is characterized by an incidence of 1 in 4000, maps to chromosome 17 and encodes a protein designated Neurofibromin, which is a large protein with a GAP domain. Genetic linkage studies of both sporadic and familial tumors suggest that NF2 is caused by inactivation of a tumor suppressor gene that maps on chromosome 22q12 and encodes a 595 amino acid protein whose function appears to be mediated by interaction with the cytoskeleton. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NF2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of NF2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of NF2 of human origin |
| Entrez Gene | 4771 |
|---|
Application
Western blot analysis of NF2 expression in non-transfected 293T: (A), mouse NF2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic staining.
Application
Western blot analysis of NF2 expression in KNRK whole cell lysate.| Application Notes | recommended for detection of NF2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
