Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of IκB-α expression in HeLa (A), Jurkat (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of IκB-α expression in HeLa (A), Jurkat (B), A-431 (C), HL-60 (D), NIH/3T3 (E) and KNRK (F) whole cell lysates.| Product Name | IκB-α Antibody (C-21) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of IκB-α of human origin |
| Modification Notes | On the basis of both functional and structural considerations, members of the I˚B family of proteins can be divided into four groups. The first of these groups, I˚B-å, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to ˚B sites, suggesting that the I˚B-å family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the I˚B family is represented by a protein designated I˚B-∫. The third group of I˚B proteins is represented by I˚B-©, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional I˚B family member, I˚B-é, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IκB-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of I&kappa B-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of IκB-α of human origin |
| Entrez Gene | 4792 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of IκB-α expression in HeLa (A), Jurkat (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of IκB-α expression in HeLa (A), Jurkat (B), A-431 (C), HL-60 (D), NIH/3T3 (E) and KNRK (F) whole cell lysates.| Application Notes | recommended for detection of IκB-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
