Application
Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of JAK2 expression in K-562 (A), CCRF-CEM (B), Daudi (C), MEG-01 (D) and MCF7 (E) whole cell lysates.Application
Western blot analysis of JAK2 expression in CCRF-CEM (A), NIH/3T3 (B), HEL 92.1.7 (C), K-562 (D), Jurkat (E) and TF-1 (F) whole cell lysates.| Product Name | JAK2 Antibody (HR-758) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of JAK2 of mouse origin |
| Modification Notes | JAK2 (Janus kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of JAK2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of JAK2 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of JAK2 of mouse origin |
| Entrez Gene | 3717 |
|---|
Application
Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of JAK2 expression in K-562 (A), CCRF-CEM (B), Daudi (C), MEG-01 (D) and MCF7 (E) whole cell lysates.
Application
Western blot analysis of JAK2 expression in CCRF-CEM (A), NIH/3T3 (B), HEL 92.1.7 (C), K-562 (D), Jurkat (E) and TF-1 (F) whole cell lysates.| Application Notes | recommended for detection of JAK2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
