Application
Western blot analysis of NAT-8B expression in non-transfected: (A) and human NAT-8B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NAT-8 expression in non-transfected: (A) and mouse NAT-8 transfected: (B) 293T whole cell lysates and rat liver tissue extract (C).| Product Name | NAT-8B Antibody (V-11) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of NAT-8B of rat origin |
| Modification Notes | Acetyltransferases and deacetylases are protein groups most often associated with oncogenesis and cell cycle regulation. NAT-8B (N-acetyltransferase 8B), also known as CML2 (camello-like protein 2), is a 227 amino acid single-pass membrane protein that is implicated in gastrulation regulation. A member of the camello family, NAT-8B contains one N-acetyltransferase domain and is encoded by a gene that maps to human chromosome 2p13.2. The NAT-8B gene is susceptible to a nonsense mutation at Serine 16, which leads to a stop codon and subsequently, a non-functional protein that is truncated in length. Similarly, a nonsense mutation at Glutamine 168 is thought to lead to a non-functional protein, as it causes the N-acetyltransferase to become disrupted. Human chromosome 2 consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2 including Harlequin icthyosis, sitosterolemia and Alström syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NAT-8B of rat origin by WB, IP, IF and ELISA non cross-reactive with NAT-8L: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of NAT-8B of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of NAT-8B of rat origin |
Application
Western blot analysis of NAT-8B expression in non-transfected: (A) and human NAT-8B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NAT-8 expression in non-transfected: (A) and mouse NAT-8 transfected: (B) 293T whole cell lysates and rat liver tissue extract (C).| Application Notes | recommended for detection of NAT-8B of rat origin by WB, IP, IF and ELISA non cross-reactive with NAT-8L: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
