Application
Western blot analysis of LIME expression in non-transfected: (A) and human LIME transfected: (B) 293 whole cell lysates.| Product Name | LIME Antibody (FL-269) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-269 representing full length LIME of mouse origin |
| Modification Notes | Lck-interacting molecule (LIME) is a 295 amino acid transmembrane adaptor protein primarily expressed in hematopoietic and lung cells. LIME has a short extracellular domain and a cytoplasmic tail containing five tyrosine-based motifs. LIME becomes tyrosine-phosphorylated after the CD4 or CD8 co-receptors cross-link. The phosphorylated LIME interacts with Lck, the Src fam-ily kinase, and Csk, its negative regulator. LIME is expressed during the early and late stages of T cell activation and appears to be involved in regulation of T cell activation by co-receptors. It may be involved in activation of the ERK and JNK (both are part of the mitogen-activated protein kinase family) pathways in T cells. BCR-mediated B cell activation may also involve LIME. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LIME of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-269 representing full length LIME of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-269 representing full length LIME of mouse origin |
| Entrez Gene | 54923 |
|---|
Application
Western blot analysis of LIME expression in non-transfected: (A) and human LIME transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of LIME of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
