Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts.Application
Western blot analysis of Cbl expression in K-562 whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of Cbl expression in 3T3-L1 (A), NIH/3T3 (B) and IB4 (C) whole cell lysates.Application
Western blot analysis of Cbl p120 expression in K-562 (A), 3611-RF (B) and SK-BR-3 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Cbl Antibody (C-15) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Cbl of human origin |
| Modification Notes | Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias; c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4;11) or t(11;14) abnormalities. ******Background shortened to fit on datasheet, per Susan******* |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of normal and rearranged forms of Cbl of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Cbl of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Cbl of human origin |
| Entrez Gene | 867 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts.
Application
Western blot analysis of Cbl expression in K-562 whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of Cbl expression in 3T3-L1 (A), NIH/3T3 (B) and IB4 (C) whole cell lysates.
Application
Western blot analysis of Cbl p120 expression in K-562 (A), 3611-RF (B) and SK-BR-3 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of normal and rearranged forms of Cbl of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
