Application
Western blot analysis of PSPC1 expression in non-transfected: (A) and human PSPC1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PSPC1 expression in non-transfected 293T: (A), human PSPC1 transfected 293T: (B), HeLa (C), T24 (D), WI 38 (E) and F9 (F) whole cell lysates.Application
Western blot analysis of PSPC1 expression in HeLa whole cell lysate (A) and mouse thymus tissue extract (B).| Product Name | PSPC1 Antibody (T-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of PSPC1 of human origin |
| Modification Notes | PSPC1 (paraspeckle component 1), also known as PSP1, is a 523 amino acid protein that localizes to both the cytoplasm and the nuclear matrix and contains two RRM (RNA recognition motif) domains. Expressed in liver, kidney, pancreas, heart, brain, placenta and skeletal muscle, PSPC1 is able to form heterodimers with p54/nrb and functions to regulate androgen receptor-mediated gene transcription activity, specifically in sertoli cell lines. PSPC1 exists as two alternatively spliced isoforms, designated alpha and beta, which are subject to DNA damage-dependent phosphorylation, probably by ATR or ATM. The gene encoding PSPC1 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PSPC1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with PSP1-β also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of PSPC1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of PSPC1 of human origin |
| Entrez Gene | 55269 |
|---|
Application
Western blot analysis of PSPC1 expression in non-transfected: (A) and human PSPC1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PSPC1 expression in non-transfected 293T: (A), human PSPC1 transfected 293T: (B), HeLa (C), T24 (D), WI 38 (E) and F9 (F) whole cell lysates.
Application
Western blot analysis of PSPC1 expression in HeLa whole cell lysate (A) and mouse thymus tissue extract (B).| Application Notes | recommended for detection of PSPC1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with PSP1-β also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
