Application
Western blot analysis of RGS1 expression in BJAB whole cell lysate.Application
Western blot analysis of RGS1 expression in SP2/0 whole cell lysate.| Product Name | RGS1 Antibody (U-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RGS1 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Several Gå GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS 2, RGS4, RGS 7, RGS 9, RGS10 and GAIP (Gå-interacting protein). Each of these proteins has been shown to deactivate specific Gå isoforms by increasing the rate at which they convert GTP to GDP. RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Gå i, Gå o, and Gå t, but not Gå s. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RGS1 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of RGS1 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 5996 |
|---|
Application
Western blot analysis of RGS1 expression in BJAB whole cell lysate.
Application
Western blot analysis of RGS1 expression in SP2/0 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
