Application
Western blot analysis of CIR expression in K-562 whole cell lysate.| Product Name | CIR Antibody (L-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CIR of human origin by WB, IP and ELISA |
| Modification Notes | Recombination signal binding protein Jk (RBP-Jk), also designated KBF2 or CBF1, is the mammalian homolog of the Drosophila Suppressor of Hairless [Su(H)], a protein involved in the development of the peripheral nervous system. RBP-Jk is ubiquitously expressed in mammalian tissues and is involved in the regulation of gene expression. RBP-Jk has been shown to directly interact with the intracellular domain of the cell surface receptor Notch1. Proteolytically cleaved Notch1 translocates to the nucleus, where it binds to DNA-bound RBP-Jk and activates transcription of target genes. CIR (for CBF1 interacting corepressor) serves as a linker between RBP-Jk and the histone deacetylase complex by binding to SAP30 and to histone deacetylase. CIR binding to RBP-Jk results in transcriptional repression of Notch 1 target genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CIR of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of CIR of human origin by WB, IP and ELISA |
| Entrez Gene | 9541 |
|---|
Application
Western blot analysis of CIR expression in K-562 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
