Application
Western blot analysis of ARL8 expression in HL-60 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | ARL8 Antibody (E-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ARL8 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | ADP-ribosylation factors (ARFs) are highly conserved guanine nucleotide binding proteins that enhance the ADP-ribosyltransferase activity of Cholera Toxin. ARF’s are important in eukaryotic vesicular trafficking pathways, and they play an essential role in the activation of phospholipase D (PC-PLD). ARL8 (ADP-ribosylation factor-like protein 8), also known as ARL5B, is a 179 amino acid protein that belongs to the RAS superfamily of regulatory GTPases. Human ARL8 shares 100% identity with mouse Arl8 and 71% identity with the Drosophila homolog. Expressed in brain, heart, lung, cartilage and kidney, but not in spleen, ARL8 is most related to ARL5, with which it shares 80% protein sequence identity. Two isoforms of ARL8 exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ARL8 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ARL8 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 221079 |
|---|
Application
Western blot analysis of ARL8 expression in HL-60 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
