Application
Western blot analysis of ADK expression in non-transfected 293T: (A) and mouse ADK transfected 293T: (B) whole cell lysates and rat liver tissue extract (C).| Product Name | ADK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 63-362 mapping at the C-terminus of ADK of human origin |
| Modification Notes | Adenosine kinase (ATP:adenosine 5-prime-phosphotransferase), or ADK, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides (1-3). Adenosine, an extracellular signaling molecule, has widespread effects on the cardiovascular, nervous, respiratory, and immune systems with increased concentration at sites of tissue injury and inflammation (1,3,4). Adenosine is an efficient inhibitor of neuronal activity with the ability to suppress seizure activity in various animal models of epilepsy (2). The human ADK gene maps to chromosome 10q11-q24 and encodes 2 ADK transcripts that encode a 345-amino acid form and a 362-amino acid form of the enzyme (1, 5). These 2 alternately spliced forms differ only at the 5-prime end, where the first four encoded residues of the short form are replaced by 21 residues in the long form (1). When expressed, both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity (1). ADK is fully active under dilute conditions, but tends to form soluble aggregates at higher concentrations, which results in inactivation of the enzyme (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ADK long and short isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 63-362 mapping at the C-terminus of ADK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 63-362 mapping at the C-terminus of ADK of human origin |
| Entrez Gene | 132 |
|---|
Application
Western blot analysis of ADK expression in non-transfected 293T: (A) and mouse ADK transfected 293T: (B) whole cell lysates and rat liver tissue extract (C).| Application Notes | recommended for detection of ADK long and short isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
