Application
Antibodies tested include p-Adducin (Ser 726)-R: (A,B) and Adducin α (H-100): (C,D).Application
Antibodies tested include p-Adducin (Ser 662)-R: (A,B) and Adducin α (H-100): (C,D).Application
Western blot analysis of Adducin α expression in non-transfected 293T: (A), mouse Adducin α transfected 293T: (B) and T98G (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing membrane and cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoskeletal staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of Adducin α expression in SK-N-MC (A) and T98G (B) whole cell lysates and rat spleen tissue extract (C).| Product Name | Adducin α Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 581-680 of Adducin α of human origin |
| Modification Notes | Adducins are a family of cytoskeleton proteins encoded by three genes (å, ∫, ©). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and Adducins are central in the cortical actin network organization. Adducin å is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin å and Adducin © is ubiquitous in contrast to the restricted expression of Adducin ∫. Adducin ∫ is expressed at high levels in brain and hematopoietic tissues, such as bone marrow, in humans, and in spleen in mice. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Adducin α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 581-680 of Adducin &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 581-680 of Adducin α of human origin |
| Entrez Gene | 118 |
|---|
Application
Antibodies tested include p-Adducin (Ser 726)-R: (A,B) and Adducin α (H-100): (C,D).
Application
Antibodies tested include p-Adducin (Ser 662)-R: (A,B) and Adducin α (H-100): (C,D).
Application
Western blot analysis of Adducin α expression in non-transfected 293T: (A), mouse Adducin α transfected 293T: (B) and T98G (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing membrane and cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoskeletal staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of Adducin α expression in SK-N-MC (A) and T98G (B) whole cell lysates and rat spleen tissue extract (C).| Application Notes | recommended for detection of Adducin α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
