Application
Western blot analysis of ME1 expression in mouse liver tissue extract.Application
Western blot analysis of ME1 expression in non-transfected: (A) and mouse ME1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ME1 expression in non-transfected: (A) and mouse ME1 transfected: (B) 293T whole cell lysates.| Product Name | ME1 Antibody (H-47) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 34-80 mapping near the N-terminus of ME1 of human origin |
| Modification Notes | ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through its ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. The expression of ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ME1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 34-80 mapping near the N-terminus of ME1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 34-80 mapping near the N-terminus of ME1 of human origin |
| Entrez Gene | 4199 |
|---|
Application
Western blot analysis of ME1 expression in mouse liver tissue extract.
Application
Western blot analysis of ME1 expression in non-transfected: (A) and mouse ME1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ME1 expression in non-transfected: (A) and mouse ME1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ME1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
