Application
Antibodies tested include p-Rsk (C-5): (A,B,C) and Ribosomal Rsk-1 (C-21): (D,E,F).Application
Antibodies tested include p-Rsk (Thr 359/Ser 363)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).Application
Antibodies tested include p-Rsk (Ser 227)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).Application
Antibodies tested include p-Rsk-1/2/4 (Ser 363)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).Application
Antibodies tested include p-Rsk (Ser 380)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).Application
Antibodies tested include p-Rsk (Ser 227)-R: (A,B) and Rsk-1 (C-21): (C,D).Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Blots were probed with p-Rsk-1/2/4 (Ser 363)-R: preincubated with cognate phosphorylated (A) and unphosphorylated (B) peptide and Rsk-1 (C-21): (C).Application
Cytoplasmic immunofluorescence staining of methanol-fixed NIH/3T3 cells.Application
Antibodies tested include Rsk-1 (C-21): (A-E) and Rsk-1 (C-21)-G: (F-J).Application
Antibodies tested include Rsk-1 (C-21): (A,B), p-Rsk (Ser 376): (C,D) and p-Rsk (Ser 376)-R: (E,F).Application
Antibodies tested include Rsk-1 (C-21): (A,B) and p-Rsk-1/2 (Thr 359/Ser 363)-R: (C,D).| Product Name | Rsk-1 Antibody (C-21) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Rsk-1 of human origin |
| Modification Notes | The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development. The family of ribosomal S6 kinases includes p70 S6 kinase and p70 S6 kinase ∫, which are thought to have similar regulatory functions. MSK1 (also designated RLPK) is a novel Rsk-related protein, which, like the p90 Rsk family members, contains two non-identical complete kinase catalytic domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Rsk-1 of mouse, rat, human, mink and canine origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Rsk-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Rsk-1 of human origin |
| Entrez Gene | 6195 |
|---|
Application
Antibodies tested include p-Rsk (C-5): (A,B,C) and Ribosomal Rsk-1 (C-21): (D,E,F).
Application
Antibodies tested include p-Rsk (Thr 359/Ser 363)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).
Application
Antibodies tested include p-Rsk (Ser 227)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).
Application
Antibodies tested include p-Rsk-1/2/4 (Ser 363)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).
Application
Antibodies tested include p-Rsk (Ser 380)-R: (A,B,C) and Rsk-1 (C-21): (D,E,F).
Application
Antibodies tested include p-Rsk (Ser 227)-R: (A,B) and Rsk-1 (C-21): (C,D).
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Blots were probed with p-Rsk-1/2/4 (Ser 363)-R: preincubated with cognate phosphorylated (A) and unphosphorylated (B) peptide and Rsk-1 (C-21): (C).
Application
Cytoplasmic immunofluorescence staining of methanol-fixed NIH/3T3 cells.
Application
Antibodies tested include Rsk-1 (C-21): (A-E) and Rsk-1 (C-21)-G: (F-J).
Application
Antibodies tested include Rsk-1 (C-21): (A,B), p-Rsk (Ser 376): (C,D) and p-Rsk (Ser 376)-R: (E,F).
Application
Antibodies tested include Rsk-1 (C-21): (A,B) and p-Rsk-1/2 (Thr 359/Ser 363)-R: (C,D).| Application Notes | recommended for detection of Rsk-1 of mouse, rat, human, mink and canine origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
