Application
Western blot analysis of Arp2 expression in non-transfected: (A) and human Arp2 transfected: (B) 293 whole cell lysates and HeLa nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urothelial cancer showing cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic localization.Application
Western blot analysis of Arp2 expression in RAW 264.7 whole cell lysate.Application
Western blot analysis of Arp2 expression in C32 (A) and KNRK (B) whole cell lysates.| Product Name | Arp2 Antibody (H-84) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 311-394 mapping at the C-terminus of Arp2 of human origin |
| Modification Notes | Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Arp2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 311-394 mapping at the C-terminus of Arp2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 311-394 mapping at the C-terminus of Arp2 of human origin |
| Entrez Gene | 10097 |
|---|
Application
Western blot analysis of Arp2 expression in non-transfected: (A) and human Arp2 transfected: (B) 293 whole cell lysates and HeLa nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urothelial cancer showing cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic localization.
Application
Western blot analysis of Arp2 expression in RAW 264.7 whole cell lysate.
Application
Western blot analysis of Arp2 expression in C32 (A) and KNRK (B) whole cell lysates.| Application Notes | recommended for detection of Arp2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
