Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.Application
Western blot analysis of WASP expression in Ramos whole cell lysate.Application
Western blot analysis of WASP expression in non-transfected: (A) and human WASP transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed BJAB cells showing cytoplasmic staining.Application
Western blot analysis of WASP expression in BJAB (A) and MOLT-4 (B) whole cell lysates.| Product Name | WASP Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-250 of WASP of human origin |
| Modification Notes | The Wiskott-Aldrich syndrome (WAS) is a disorder that results from a monogenic defect that has been mapped to the short arm of the X chromosome. WAS is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity and a propensity for lymphoproliferative disease. The gene that is mutated in the syndrome encodes a proline-rich protein of unknown function designated WAS protein (WASP). A clue to WASP function came from the observation that T cells from affected males had an irregular cellular morphology and a disarrayed cytoskeleton suggesting the involvement of WASP in cytoskeletal organization. Close examination of the WASP sequence revealed a putative Cdc42/Rac interacting domain, homologous with those found in PAK65 and ACK. Subsequent investigation has shown WASP to be a true downstream effector of Cdc42. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of WASP and, to a lesser extent, N-WASP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-250 of WASP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-250 of WASP of human origin |
| Entrez Gene | 7454 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.
Application
Western blot analysis of WASP expression in Ramos whole cell lysate.
Application
Western blot analysis of WASP expression in non-transfected: (A) and human WASP transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed BJAB cells showing cytoplasmic staining.
Application
Western blot analysis of WASP expression in BJAB (A) and MOLT-4 (B) whole cell lysates.| Application Notes | recommended for detection of WASP and, to a lesser extent, N-WASP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
