Application
Western blot analysis of CFTR expression in MH-S (A) and AMJ2-C8 (B) whole cell lysates.Application
Western blot analysis of CFTR expression in Calu-3 whole cell lysate (A) and CFTR-transfected BHK cells (B).Application
Immunofluorescence staining of methanol-fixed A549 cells showing membrane localization.| Product Name | CFTR Antibody (H-182) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-182 mapping at the N-terminus of CFTR of human origin |
| Modification Notes | CFTR, for cystic fibrosis transmembrane conductance regulator, is a cyclic adenosine monophosphate (cAMP)-regulated chloride channel protein. CFTR belongs to the MDR subfamily within the ATP-binding transport protein family. It has two transmembrane domains (TMDs), two nucleotide binding domains (NBDs) and one regulatory domain. Mutations of CFTR are associated with cystic fibrosis (CF), a disease characterized by chronic bronchopulmonary disease, elevated sweat electrolytes and insufficient pancreatic function. CFTR mutations can also result in congenital bilateral absence of vas deferens (CBAVD), a form of male sterility that a majority of male CF patients exhibit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CFTR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-182 mapping at the N-terminus of CFTR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-182 mapping at the N-terminus of CFTR of human origin |
| Entrez Gene | 1080 |
|---|
Application
Western blot analysis of CFTR expression in MH-S (A) and AMJ2-C8 (B) whole cell lysates.
Application
Western blot analysis of CFTR expression in Calu-3 whole cell lysate (A) and CFTR-transfected BHK cells (B).
Application
Immunofluorescence staining of methanol-fixed A549 cells showing membrane localization.| Application Notes | recommended for detection of CFTR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
