Application
Western blot analysis of CD14 expression in non-transfected: (A) and mouse CD14 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD14 expression in non-transfected: (A) and human CD14 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD14 expression in BJAB whole cell lysate.Application
Western blot analysis of mouse recombinant CD14.| Product Name | CD14 Antibody (M-305) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 17-321 mapping within an internal region of CD14 of mouse origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the “adapter molecule,” lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14 (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of CD14 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 17-321 mapping within an internal region of CD14 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 17-321 mapping within an internal region of CD14 of mouse origin |
| Entrez Gene | 929 |
|---|
Application
Western blot analysis of CD14 expression in non-transfected: (A) and mouse CD14 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD14 expression in non-transfected: (A) and human CD14 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD14 expression in BJAB whole cell lysate.
Application
Western blot analysis of mouse recombinant CD14.| Application Notes | recommended for detection of CD14 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
