Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.Application
Western blot analysis of Rab 5B expression in MDCK whole cell lysate.Application
Western blot analysis of Rab 5B expression in non-transfected 293T: (A), human Rab 5B transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of Rab 5B expression in HeLa (A), NIH/3T3 (B) and MDCK (C) whole cell lysates.| Product Name | Rab 5B Antibody (A-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the C-terminus of Rab 5B of human origin |
| Modification Notes | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rab 5B of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the C-terminus of Rab 5B of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the C-terminus of Rab 5B of human origin |
| Entrez Gene | 5869 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.
Application
Western blot analysis of Rab 5B expression in MDCK whole cell lysate.
Application
Western blot analysis of Rab 5B expression in non-transfected 293T: (A), human Rab 5B transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of Rab 5B expression in HeLa (A), NIH/3T3 (B) and MDCK (C) whole cell lysates.| Application Notes | recommended for detection of Rab 5B of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
