Application
Western blot analysis of GCKR expression in non-transfected: (A) and mouse GCKR transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of GCKR expression in mouse liver extract.| Product Name | GCKR Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of glucokinase regulator (GCKR) of human origin |
| Modification Notes | Glucokinase (also designated hexokinase IV or GCK) plays a key role in the regulation of glucose-induced insulin secretion. GCK is expressed in pancreatic ∫ cells, where it functions as a glucose sensor, determining the “set point” for insulin secretion. GCK is also expressed in the liver, where it catalyzes the first step in the disposal of glucose. A lack of glucokinase activity leads to reduced insulin secretion and hyperglycemia and has been implicated as a cause for maturity onset diabetes of the youth (MODY). Heterozygous point mutations in the gene encoding GCK have been detected in individuals suffering from MODY. GCK is regulated by GCKR (glucokinase regulatory protein). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GCKR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of glucokinase regulator (GCKR) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of glucokinase regulator (GCKR) of human origin |
| Entrez Gene | 2646 |
|---|
Application
Western blot analysis of GCKR expression in non-transfected: (A) and mouse GCKR transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of GCKR expression in mouse liver extract.| Application Notes | recommended for detection of GCKR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
